多浪羊Lin28A基因克隆及其在初情期启动过程中的表达研究

doi:10.11843/j.issn.0366-6964.2019.01.010

Abstract

Abstract:

The objectives of the present study were to clone the Lin28A cDNA of sheep, investigate its expression patterns and regulation properties during the onset of puberty in sheep. In this study, 5 Duolang sheep were slaughtered in the prepuberty (1 week before the first estrus), puberty and postpuberty (1 week after the first estrus),respectively,hypothalamus, pituitary and ovarian tissues were collected. The Lin28A cDNA of these tissues was cloned, the homology of the amino acids of Lin28A between Duolang sheep and other species were compared by DNAman 6.0. The expression patterns of Lin28A, let-7a and let-7b in hypothalamus, pituitary and ovary during the puberal transition in Duolang sheep were detected by real-time PCR.The results showed that the fragment of Lin28A cDNA was 3 581 bp which contained 2 963 bp of 3' UTR and 618 bp of the coding region.The deduced protein sequence of the Lin28A coding region shared 87.56%, 88.52%, 92.68%, 89.95%,76.10% and 61.84% to that of human, house mouse, cattle, sheep, original chicken and zebrafish. The expression of Lin28A was significantly decreased in the hypothalamus and ovary from prepuberty to puberty (P<0.05), the expression levels of let-7a and let-7b did not change significantly (P>0.05).The results provided a foundation for revealing the function of Lin28A gene and its role in the onset of puberty in sheep.

CLC Number:

S826.2

XING Feng, GAO Qinghua, QI Xin, LI Qingjin, LI Chuang. Cloning and Expression of Lin28A Gene in the Onset of Puberty in Duolang Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(1): 78-85.

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Cloning and Expression of Lin28A Gene in the Onset of Puberty in Duolang Sheep

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